| Forward Primers | EcoRI restriction sequence and the initiating methionine codon are indicated in underline and bold, respectively. |
| Met1 primer | 5′-GGGGGAATTCATGAATATATCGAACAGTC-3′ |
| Leu180 primer | 5′-GGGGGAATTCATGCTTGCTGCTTTTGATGAT-3 |
| Ala557 primer | 5′-GGGGGAATTCATGGCGCAGCAGCTGACT-3′ |
| Reverse Primers | HindIII restriction sequence and the stop codon are indicated in underline and bold, respectively |
| Glu641 Primer | 5′-GGGGGGATCCTCATTCATCCTCTCCGGATAA-3 |
| Glu556 primer | 5′-GGGGGGATCCTCATTCGGTCAGATCAAAAATA-3 |
| Glu179 primer | 5′-GGGGGGATCCTCAATGTTCATCATAACCCGCCA-3′ |
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