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. 2007 Nov 28;2(11):e1227. doi: 10.1371/journal.pone.0001227

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Lévesque et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Expression of TGF-β1 determined by whole-mount in situ hybridization in axolotl forelimbs. Limbs were amputated distally through radius/ulna. Samples were fixed at various times after amputation: 6 hours (6 h), 48 hours (48 h), early bud (EB), late bud (LB) and early differentiation (ED) stage. TGF-β1 expression is found as a dark purple precipitate. The 3 stages on the upper row are part of the preparation phase of limb regeneration. The 2 stages on the lower row are part of the redevelopment phase of limb regeneration. B) Northern blot showing expression of TGF-β1 at various stages of forelimb regeneration. TGF-β1 was detected as a 3.4 Kb transcript. RNA was extracted from regenerating blastemas at various times after amputation: 6 hours (6 h), 24 hours (24 h), 48 hours (48 h), early bud (EB), late bud (LB), palette (PAL) and early differentiation (ED) stage. T = 0: RNA was extracted from an unamputated mature limb. The dotted line marks a distinction between regeneration stages included in the preparation phase and those included in the redevelopment phase of regeneration.