Supplemental Table 1.

Effects of Running Wheels on Sleep/Wake Behavior.

DARK PHASE
	% of hour		Mean bout duration (sec)		Number of bouts
	WR	WL	WR	WL	WR	WL
Wake
WT	86.0 ± 1.5**	71.5 ± 1.9	1697 ± 267 **	435 ± 26	26 ± 3**	76 ± 4
KO	78.8 ± 1.0**	66.3 ± 1.3	366 ± 26 **	210 ± 11	97 ± 6 **	139 ± 6
NREM
WT	12.7 ± 1.3**	25.4 ± 1.8	228 ± 21 *	162 ± 17	25 ± 3**	75.2 ± 5
KO	15.2 ± 1.0**	26.6 ± 1.3	119 ± 7*	99 ± 5	57 ± 6**	121.2 ± 7
REM
WT	1.2 ± 0.2**	3.1 ± 0.3	59 ± 5	59 ± 2	9 ± 1**	24.4 ± 3
KO	1.3 ± 0.2**	4.1 ± 0.4	44 ± 4 **	55 ± 3	13 ± 1 **	31.5 ± 2.5
Cataplexy
KO	4.7 ± 0.5**	2.9 ± 0.4	52 ± 4*	63 ± 6	40 ± 4**	18 ± 2

LIGHT PHASE
	% of hour		Mean bout duration (sec)		Number of bouts
	WR	WL	WR	WL	WR	WL
Wake
WT	34.9 ± 1.5	37.9 ± 1.4	123 ± 27	106 ± 12	128 ± 10	161 ± 15
KO	35.3 ± 1.0	36.6 ± 0.9	90 ± 11	97 ± 10	170 ± 10	166 ± 6
NREM
WT	57.0 ± 1.3	54.6 ± 0.9	196 ± 38	151 ± 30	130 ± 10	161 ± 15
KO	56.3 ± 1.0	55.3 ± 0.8	145 ± 26	145 ± 18	173 ± 9	166 ± 7
REM
WT	8.0 ± 0.3	7.4 ± 0.8	64 ± 5	72 ± 19	54 ± 2	47 ± 4
KO	8.2 ± 0.4	7.6 ± 0.4	57 ± 57	69 ± 30	74 ± 4	64 ± 4
Cataplexy
KO	0.3 ± 0.1	0.4 ± 0.1	56 ± 23	92 ± 60	2 ± 1	2 ± 1

Running wheels increased and consolidated wakefulness during the dark period but had no effects on behavior during the light period. The total amount of wakefulness increased in both WT and orexin KO mice, but KO mice continued to have relatively short bouts of wakefulness even with running wheels. In orexin KO mice, running wheels doubled the number of bouts of cataplexy. *, p < 0.05; **, p < 0.01.