Figure 4.

LMA2 is less efficient at promoting vacuole fusion than LMA1. (A) Aliquots were removed from the equivalent of ten regular fusion assays containing equal amounts of either LMA1 or LMA2 and placed on ice. Each aliquot was assayed for alkaline phosphatase activity. (B) Vacuoles were incubated with Sec18p and LMA1 or LMA2 containing equivalent amounts of I₂^B. The data are the average of three experiments ± SD and are shown as a percentage of the maximal fusion signal (A, 0.98 units; B, 1.05 units).