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LMA2 supports the fusion of vacuoles lacking protease B. Fresh, salt-washed vacuoles were incubated for 2 hr at 0°C (A) or 27°C (B and C) in the presence of Sec18p (B) or Sec18p and LMA2 (A and C). Vacuoles were stained with FM4-64 and visualized by fluorescence microscopy. (Bar = 4 μm.)
