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. 1997 May 27;94(11):5582–5587. doi: 10.1073/pnas.94.11.5582

Figure 7.

Figure 7

Yeast cytosol contains heat-stable activities other than LMA2. (A) Frozen, salt-washed vacuoles were incubated with ATP alone, 30 μg of I2B-wild-type cytosol (K91-1A), 1.2 μg of I2B-wild-type HCS, or 1.2 μg of I2B-deletion HCS. Data were normalized to the maximal (cytosol) signal (1.25 units) and the average of four experiments is shown ± SD. (B) Fresh vacuoles that had not been salt-washed were preincubated with preimmune or anti-Sec18p IgG. After removal of the antibody, vacuoles were incubated with ATP and 30 μg of cytosol, either with or without immunodepletion of Sec17p or Sec18p, or with 1.2 μg of HCS from wild-type or I2B-deletion cytosol. The average of two experiments is shown.