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. Author manuscript; available in PMC: 2008 Sep 26.
Published in final edited form as: J Control Release. 2007 Jul 19;122(2):165–172. doi: 10.1016/j.jconrel.2007.07.004

Table 1.

Chromatographic conditions employed for the analysis of compounds studied in this work.

N° Compound Detection mode a Column b Mobile phase c
5 Tetra-N-methylammonium Chloride ED CG14 + CS14 HMSA 5mM + ACN 5%
6 Tetra-N-ethylammonium Chloride ED CG14 + CS14 HMSA 30mM + ACN 5%
7 Tetra-N-proylammonium Chloride ED CG14 + CS14 HMSA 30mM + ACN 20%
8 Choline Chloride ED CG14 + CS14 HMSA 5mM + ACN 20%
9 Butyrylthiocholine Chloride ED CG14 + CS14 HMSA 30mM + ACN 20%
10 Carbamoylcholine Chloride ED CG14 + CS14 HMSA 5mM + ACN 20%
11 Neostigmine Bromide UV (260nm) Zorbax CN [Acetate buffer pH=5.2 + HESA 10mM] 30% + ACN 70%
12 Pyridostigmine Bromide UV (275nm) Zorbax CN [Acetate buffer pH=5.2+ HESA 10mM] 40% + ACN 60%
a

ED = electrochemical detection; UV = ultraviolet detection.

b

CG = Column guard; CS = analytical column.

c

HMSA = Methanesulfonic acid, ACN = Acetonitrile, HESA = heptansulfonic acid sodium salt