Table 2.
Functional annotation clustering of genes differentially expressed in R6/1 Huntington’s disease transgenic mice at a significance level of p<0.05
| Probe ID | Gene Description | Gene ID | Fold change R6/1 | Fold change Human* |
|---|---|---|---|---|
| Glycosyltransferases | ||||
| 1423305_at | exostoses (multiple)-like 1 | Extl1 | 0.776 | NC |
| 1434703_at | exostoses (multiple)-like 3 | Extl3 | 0.725 | 0.89 |
| 1421415_s_at | glucosaminyl (n-acetyl) transferase 2, i-branching enzyme | Gcnt2 | 0.661 | 0.94 |
| 1459522_s_at | glycogenin | Gyg1 | 1.314 | 1.22 |
| 1417435_at | like-glycosyltransferase | Large | 0.632 | 0.65 |
| 1424720_at | mannoside acetylglucosaminyltransferase 4, isoenzyme b | Mgat4b | 0.571 | 0.95 |
| 1428644_at | mannoside acetylglucosaminyltransferase 5 | Mgat5 | 0.418 | NC |
| 1434531_at | mannoside acetylglucosaminyltransferase 5, isoenzyme b | Mgat5b | 0.72 | 0.84 |
| 1451738_at | o-linked n-acetylglucosamine (glcnac) transferase | Ogt | 1.36 | 0.85 |
| 1437999_x_at | phosphatidylinositol glycan, class q | Pigq | 0.717 | NC |
| 1422502_at | poly (adp-ribose) polymerase family, member 1 | Parp1 | 0.776 | 1.10 |
| 1428931_a_at | poly (adp-ribose) polymerase family, member 6 | Parp6 | 0.627 | 1.39 |
| 1426618_a_at | protein o-linked mannose beta1,2-n-acetylglucosaminyltransferase | Pomgntl | 0.781 | 1.12 |
| 1460704_at | radical fringe gene homolog (drosophila) | Rfng | 0.799 | 1.05 |
| 1419063_at | udp galactosyltransferase 8a | Ugt8a | 0.425 | NC |
| 1418080_at | udp-gal:betaglcnac beta 1,4- galactosyltransferase, polypeptide 2 | B4galt2 | 0.568 | NC |
| 1434135_at | udp-galnac:betaglcnac beta 1,3-galactosaminyltransferase, polypeptide 2 | B3galnt2 | 0.738 | A |
| 1420852_a_at | udp-glcnac:betagal beta-1,3-n-acetylglucosaminyltransferase 1 | B3gnt1 | 0.495 | 0.59 |
| 1418195_at | udp-n-acetyl-alpha-d-galactosamine acetylgalactosaminyltransferase 10 | Galnt10 | 0.807 | 1.31 |
| 1454780_at | udp-n-acetyl-alpha-d-galactosamine acetylgalactosaminyltransferase-like 4 | Galntl4 | 0.489 | A |
| Ganglioside Biosynthesis / Sialyltransferases | ||||
| 1449468_at | sialyltransferase 7 | St6galnac5 | 0.806 | 0.62 |
| 1421891_at | st3 beta-galactoside alpha-2,3-sialyltransferase 2 | St3gal2 | 0.595 | 0.88 |
| 1449198_a_at | st3 beta-galactoside alpha-2,3-sialyltransferase 5 | St3gal5 | 0.688 | 0.83 |
| 1420377_at | st8 alpha-n-acetyl-neuraminide alpha-2,8-sialyltransferase 2 | St8sia2 | 0.419 | 0.93 |
| 1451008_at | st8 alpha-n-acetyl-neuraminide alpha-2,8-sialyltransferase 3 | St8sia3 | 0.324 | 0.58 |
| Sphingolipid Metabolism | ||||
| 1434034_at | ceramide kinase | Cerk | 0.793 | 1.07 |
| 1454078_a_at | galactose-3-o-sulfotransferase 1 | Gal3st1 | 0.758 | 1.05 |
| 1422341_s_at | lysophospholipase 3 | Lypla3 | 1.208 | NC |
| 1437513_a_at | serine incorporator 1 | Serinc1 | 1.383 | 0.81 |
| 1422779_at | sphingomyelin phosphodiesterase 3, neutral | Smpd3 | 0.489 | 0.92 |
| Sulfotransferase Activity | ||||
| 1428140_at | 3-oxoacid coA transferase 1 | Oxct1 | 0.645 | 0.73 |
| 1449147_at | carbohydrate (keratan sulfate gal-6) sulfotransferase 1 | Chst1 | 0.567 | 0.83 |
| 1428902_at | carbohydrate sulfotransferase 11 | Chst11 | 0.654 | 0.87 |
| 1422758_at | carbohydrate sulfotransferase 2 | Chst2 | 0.557 | 0.84 |
| 1422739_at | heparan sulfate 2-o-sulfotransferase 1 | Hs2st1 | 0.711 | 0.82 |
| 1417293_at | heparan sulfate 6-o-sulfotransferase 1 | Hs6st1 | 0.648 | 1.09 |
| 1428367_at | n-deacetylase/n-sulfotransferase (heparan glucosaminyl) 1 | Ndst1 | 0.567 | 1.10 |
| 1421733_a_at | protein-tyrosine sulfotransferase 1 | Tpst1 | 0.573 | 1.33 |
| 1416086_at | protein-tyrosine sulfotransferase 2 | Tpst2 | 0.695 | 1.35 |
| 1448609_at | thiosulfate sulfurtransferase, mitochondrial | Tst | 0.731 | 1.58 |
Expression fold-changes represent the fold difference of geometric means between HD mice and wild-type controls at a p<0.05 significance level in the Affymetrix mouse 430 2.0 array. Functional annotation clustering was made applying the 2.1 program version of DAVID, the Database for Annotation, Visualization and Integrated Discovery, from the National Institute of Allergy and Infectious Diseases (NIAID), NIH (http://david.abcc.ncifcrf.gov).Shade boxes represent sequences reported as changed in the GLYCOv2 chip as well.
Changes for human caudate samples represent the fold difference of geometric means between HD mice and wild-type controls at a p<0.05 significance level, as reported in Supplementary Table S1 by Hodges et al. (Hodges et al., 2006).