Figure 7.

Lactate dehydrogenase (LDH) release in isolated hepatocytes. LDH release indicates that membrane integrity of hepatocytes is partly damaged during the process of isolation (day 0 = fresh isolated hepatocytes). After seeding onto collagen coated dishes, hepatocytes recover and some necrotic cells are constantly removed by a daily medium change, which is demonstrated by dropping of LDH enzyme activity to low levels in the medium after 2 days. Six different preparations of PHHs are shown.