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. 2008 Jan-Feb;14(1-2):11–19. doi: 10.2119/2007-00062.Jeschke

Table 1.

Oligonucleotide sequence for real-time PCR

Sequence, 5′to 3′ Sequence length, bp Concentration, mM Annealing temperature, °C
β-Actin Forward
Reverse
AGA GGG AAA TCG TGC GTG AC
CAA TAG TGA TGA CCT GGC CGT
138 3 62
GAPDH Forward
Reverse
GCG GGG CTC TCC AGA ACA TCA T
CCA GCC CCA GCG TCA AAG GTG
301 3 64
IL-1β Forward
Reverse
CAG GCC GCG TCA GTT GTT GT
CCG GAG CGT GCA GTT CAG TG
195 3 60
TNF-α Forward
Reverse
CGC CAC CAC GCT CTT CTG C
ACG GCG ATG CGG CTG ATG
354 2 64
IL-6 Forward
Reverse
CCC AGT ACC CCC AGG AGA AGA
GTT GGG TCA GGG GTG GTT ATT G
426 3 60
IL-10 Forward
Reverse
GAC CCA GCC CCT TGA GAA ACC T
GCC CCA AGC CCA GAG ACA AGA T
359 3 64
IFN-γ Forward
Reverse
GGG TTC TCT TGG CTG TTA CT
TTG GCT CTG CAT TAT TTT TC
166 3 58
STAT-5 Forward
Reverse
TCA TCA TCG AGA AGC AGC C
TTC CGT CAC AGA CTC TGC AC
317 3 60
α-SMA Forward
Reverse
CGT GGC TAT TCC TTC GTT AC
TGC CAG CAG ACT CCA TCC
174 3 57
CD68 Forward
Reverse
AGC CCA GGA TTC ACC AGT T
GGT TTT GTT GGG GTT CAG TAC
144 3 57
Albumin Forward
Reverse
GCTGCCATGGAGATCTGCTTGA
GCAAGTCAGCAGGCATCTCATC
174 3 53
CK18 Forward
Reverse
CCCGCTACGCCCTACAGA
GCGGGTGGTGGTCTTTTG
248 3 53