Table 1.
Sequence, 5′to 3′ | Sequence length, bp | Concentration, mM | Annealing temperature, °C | ||
---|---|---|---|---|---|
β-Actin | Forward
Reverse |
AGA GGG AAA TCG TGC GTG AC
CAA TAG TGA TGA CCT GGC CGT |
138 | 3 | 62 |
GAPDH | Forward
Reverse |
GCG GGG CTC TCC AGA ACA TCA T
CCA GCC CCA GCG TCA AAG GTG |
301 | 3 | 64 |
IL-1β | Forward
Reverse |
CAG GCC GCG TCA GTT GTT GT
CCG GAG CGT GCA GTT CAG TG |
195 | 3 | 60 |
TNF-α | Forward
Reverse |
CGC CAC CAC GCT CTT CTG C
ACG GCG ATG CGG CTG ATG |
354 | 2 | 64 |
IL-6 | Forward
Reverse |
CCC AGT ACC CCC AGG AGA AGA
GTT GGG TCA GGG GTG GTT ATT G |
426 | 3 | 60 |
IL-10 | Forward
Reverse |
GAC CCA GCC CCT TGA GAA ACC T
GCC CCA AGC CCA GAG ACA AGA T |
359 | 3 | 64 |
IFN-γ | Forward
Reverse |
GGG TTC TCT TGG CTG TTA CT
TTG GCT CTG CAT TAT TTT TC |
166 | 3 | 58 |
STAT-5 | Forward
Reverse |
TCA TCA TCG AGA AGC AGC C
TTC CGT CAC AGA CTC TGC AC |
317 | 3 | 60 |
α-SMA | Forward
Reverse |
CGT GGC TAT TCC TTC GTT AC
TGC CAG CAG ACT CCA TCC |
174 | 3 | 57 |
CD68 | Forward
Reverse |
AGC CCA GGA TTC ACC AGT T
GGT TTT GTT GGG GTT CAG TAC |
144 | 3 | 57 |
Albumin | Forward
Reverse |
GCTGCCATGGAGATCTGCTTGA
GCAAGTCAGCAGGCATCTCATC |
174 | 3 | 53 |
CK18 | Forward
Reverse |
CCCGCTACGCCCTACAGA
GCGGGTGGTGGTCTTTTG |
248 | 3 | 53 |