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. 2007 Nov 21;117(12):3730–3741. doi: 10.1172/JCI32967

Figure 6. Apoptosis and cardiomyocyte differentiation after DOX.

Figure 6

(A) CO abrogated the loss of GATA-4 DNA binding activity after DOX. PAGE showing GATA-4 DNA binding activity of nuclear protein (10 μg) by EMSA. The top arrow denotes the specific protein-DNA complex. The bottom arrow shows the nonspecific (NS) free probe. A 100-fold molar excess of cold consensus or mutant oligonucleotides confirmed the binding specificity in competition studies (data not shown). (B) Myosin heavy chain (MHC) isoforms in LV homogenates after silver staining. Western blot shows the DOX effect on myocardial troponin I and α-actinin relative to tubulin expression. (C) Top: Western blot of pAkt/Akt ratio in mouse heart before and after CO treatment and with or without DOX. Middle: Western blot of total and phospho-Bad demonstrates enhanced phosphorylation of the antiapoptotic protein in the heart after CO. Bottom: Cardiac GSK3β protein by western analysis shows an increase in pGSK3β/GSK3β ratio after CO.