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. 2007 Nov 21;117(12):3821–3832. doi: 10.1172/JCI32301

Figure 1. tPA promotes TGF-β1–mediated myofibroblast activation from renal interstitial fibroblasts.

Figure 1

NRK-49F cells were treated with 10–8 M tPA and/or 0.5 ng/ml TGF-β1 for 24 hours, unless indicated otherwise. (A and B) Western blot analyses demonstrated that tPA synergistically promoted the TGF-β1–induced α-SMA expression in a time- (A) and dose-dependent manner (B) in NRK-49F cells. (C) RT-PCR shows that tPA promoted the α-SMA mRNA expression induced by TGF-β1. (D) Blockade of TGF-β signaling abolished the synergistic effect of tPA. NRK-49F cells were treated with 10–8 M tPA and/or 0.5 ng/ml TGF-β1 in the absence or presence of the TGF-β type I receptor inhibitor SB431542 (10 μM). (E) RT-PCR shows that tPA promoted the TGF-β1–mediated mRNA induction of type I collagen. (F) Immunofluorescence staining shows that tPA promoted TGF-β1–induced type I collagen expression in NRK-49F cells. Scale bar: 20 μm.