Table 2.
Comparison of the catalytic activities of the recombinant chicken FAS domain I and its constituent subdomains with those of native chicken FAS
| Protein fractions | Specific activity, nmol per/mg
|
||
|---|---|---|---|
| AT | MT | DH | |
| Chicken FAS domain I | |||
| Crude extract | 15 | 90 | ND |
| 0–55% (NH4)SO4 | 58 | 86 | ND |
| Amylose affinity (2.5 mg) | 405 | 200 | 14 |
| Chicken FAS AT/MT-DH | 422 | 195 | 14 |
| Chicken FAS AT/MT | 400 | 220 | ND |
| Chicken FAS DH* | ND | ND | 30 |
| Native chicken FAS | 730 | 300 | 16 |
The partial activities were assayed as described in Experimental Procedures. AT, acetyl transacylase; MT, malonyl transacylase; DH, β-hydroxyacyl dehydratase; ND, not detectable.
*
This dehydratase preparation was obtained by using procedure 2 as described in Experimental Procedures.