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Acyl-binding assay
| Moles of acyl moieties bound/moles of enzyme
|
|||
|---|---|---|---|
| Total | Nonthiol | Thiol | |
| Acetyl-CoA | |||
| Chicken FAS domain I | |||
| Experiment 1 | 0.017 | 0.016 | — |
| Experiment 2 | 0.013 | 0.015 | — |
| Chicken FAS | 0.880 | 0.350 | 0.54 |
| Malonyl-CoA | |||
| Chicken FAS domain I | |||
| Experiment 1 | 0.020 | 0.021 | — |
| Experiment 2 | 0.020 | 0.020 | — |
| Chicken FAS | 1.350 | 0.620 | 0.74 |
