Table 1. Effects of various inhibitors on the first cleavage of mouse zygotes.
stage | 1-cell | 2-cell | dead | total |
AM | 1 | 44 | 1 | 46 |
vehicle | 2 | 29 | 0 | 31 |
CH | 48 | 25 | 12 | 85 |
vehicle | 16 | 66 | 2 | 84 |
CP | 14 | 135 | 1 | 150 |
vehicle | 6 | 128 | 0 | 134 |
Late pronuclei stage zygotes (around E0.75) were cultured for 24 hours in presence of following inhibitors; AM: á-amanitin 100 µg/mL, CH: cycloheximide 10 µg/mL, CP: chloramphenicol 200 µg/mL, vehicle: 1% DMSO as vehicle control. Numbers of conceptuses reaching denoted cleavage stages were counted and presented. Numbers of independent trials: 5 for AM, 8 for CH and 14 for CP.