Table 2. Effect of chloramphenicol on the second cleavage of mouse conceptuses.
stage | 2-cell | 3-cell | 4∼8-cell | total |
CP | 65 | 26 | 36 | 127 |
vehicle | 2 | 0 | 80 | 82 |
Late pronuclei stage zygotes (around E0.75) were cultured for 24 hours in presence of either 200 µg/mL of chloramphenicol (CP) or 1% of DMSO (vehicle) as vehicle control. The cleaved 2-cell conceptuses were then selected, washed and cultured in fresh medium for further 24 hours. Numbers of conceptuses reaching denoted cleavage stages were counted and presented. Number of independent trials: 15.