Figure 2.

Differential purification of other transcription regulators. (a) The protein fractions from the Bio-Rex 70 chromatographic step were analyzed for the presence of YY1, TFII-I, the RelA subunit of NF-κB, RBP-Jκ, and hRad51. In each case, the indicated bands were the only significant bands of their type observed in the whole blot and migrated at the appropriate position relative to molecular weight markers. (b) Fractions from the sedimentation analysis of the 0.6 M Bio-Rex fraction were analyzed for the presence of YY1, TFII-I, and RBP-Jκ. The protein samples were analyzed for the presence of the RelA subunit of NF-κB and for hRad51, but they were not detected in these fractions.