Figure 2.

Detection and abundance of Mcm proteins. (a) Whole-cell extracts from either the untagged parental strain PY26 (−tag) or strains containing the indicated tagged genes were subjected to immunoblot analysis. Blots were probed with the 9E10 monoclonal antibody specific for the c-myc epitope either alone (No Peptide) or after preincubation with the c-myc peptide (c-myc) or an unrelated control peptide (Control). (b) Whole-cell extracts from strains containing the indicated tagged genes (lanes 1–4) were subjected to immunoblot analysis alongside equal amounts of an extract from an untagged strain to which the indicated amounts of purified, tagged Cdc46p were added (lanes 5–11).