Figure 5.

Cdc46p and Cdc47p chromatin binding in G₁ depends on Cdc6p. (a) De novo Cdc6p synthesis in G₁ is essential for Cdc46p and Cdc47p chromatin binding. The levels of Cdc46p or Cdc47p bound to chromatin were examined in cells harboring a single CDC6 gene under the control of the MET3 promoter either in late anaphase (cdc15^ts) or after release from late anaphase into G₁ either with (−MET) or without (+MET) Cdc6p expression (10). (b) Cdc46p chromatin binding is defective in a cdc6 mutant during G₁. Cdc46p chromatin binding was examined with a wild-type and congenic cdc6–1 mutant after α factor arrest at 24°C.