Table 1.
Binding constants used for regression analysis
| Ka | DNA | Polyamide | ΔGexpt | ΔGpred (SE) | Residual | Ref. |
|---|---|---|---|---|---|---|
| 3.7 e+10 | AGTACT | IPPPIPPP | −14.26 | −12.42 (0.35) | −1.84 | 10 |
| 4.1 e+08 | AGTATT | IPPPIPPP | −11.62 | −12.05 (0.60) | 0.43 | 10 |
| 3.5 e+09 | AGTATT | IPPPPPPP | −12.88 | −13.02 (0.41) | 0.14 | 10 |
| 5.0 e+08 | AGTACT | IPPPPPPP | −11.74 | −10.88 (0.47) | −0.86 | 10 |
| 2.9 e+08 | TGTTA | IPPPPP | −11.42 | −10.68 (0.22) | −0.74 | 6 |
| 4.8 e+06 | TGACA | IPPPPP | −9.02 | −8.54 (0.38) | −0.48 | 6 |
| 1.0 e+08 | TGGTT | IIPPPP | −10.80 | −10.09 (0.23) | −0.71 | 12 |
| 1.7 e+06 | TGTTA | IIPPPP | −8.41 | −8.47 (0.42) | 0.06 | 12 |
| 1.0 e+06 | GGGTA | IIPPPP | −8.10 | −7.90 (0.43) | −0.20 | 12 |
| 1.6 e+07 | AACCA | PPPIIP | −9.72 | −10.09 (0.23) | 0.37 | 12 |
| 1.0 e+05 | TAACA | PPPIIP | −6.75 | −8.47 (0.42) | 1.72 | 12 |
| 1.0 e+05 | TACCC | PPPIIP | −6.75 | −7.90 (0.43) | 1.15 | 12 |
| 2.1 e+08 | TGTTT | IPPPPP | −11.23 | −10.68 (0.22) | −0.55 | 9 |
| 1.5 e+08 | TGTTA | IPPPPP | −11.04 | −10.68 (0.22) | −0.35 | 9 |
| 7.3 e+07 | TGTAA | IPPPPP | −10.61 | −10.68 (0.22) | 0.07 | 9 |
| 4.7 e+07 | TGTAT | IPPPPP | −10.36 | −10.68 (0.22) | 0.32 | 9 |
| 3.9 e+07 | TGATT | IPPPPP | −10.25 | −10.68 (0.22) | 0.43 | 9 |
| 2.5 e+07 | TGATA | IPPPPP | −9.99 | −10.68 (0.22) | 0.69 | 9 |
| 2.2 e+07 | TGAAA | IPPPPP | −9.91 | −10.68 (0.22) | 0.77 | 9 |
| 1.8 e+07 | TGAAT | IPPPPP | −9.79 | −10.68 (0.22) | 0.89 | 9 |
| 4.6 e+06 | AGGGA | IIIPPP | −8.99 | −9.50 (0.39) | 0.51 | 7 |
| 7.6 e+06 | TGGGT | IIIPPP | −9.29 | −9.50 (0.39) | 0.21 | 7 |
| 1.3 e+06 | TGGGC | IIIPPP | −8.25 | −7.31 (0.45) | −0.94 | 7 |
| 8.6 e+05 | AGGCA | IIIPPP | −8.01 | −6.99 (0.58) | −1.02 | 7 |
| 3.7 e+08 | AGGGAA | IIIPPPPP | −11.56 | −11.83 (0.42) | 0.27 | 7 |
| 1.4 e+07 | TGGGTC | IIIPPPPP | −9.65 | −9.64 (0.50) | −0.01 | 7 |
| 1.7 e+06 | TGGGCT | IIIPPPPP | −8.41 | −9.69 (0.50) | 1.28 | 7 |
| 2.9 e+06 | AGGCAA | IIIPPPPP | −8.72 | −9.32 (0.51) | 0.60 | 7 |
| 7.6 e+07 | TGTTA | IPPPPP | −10.64 | −10.68 (0.22) | 0.04 | 11 |
| 7.8 e+05 | AGAGT | IPPPPP | −7.95 | −8.54 (0.38) | 0.59 | 11 |
| 2.6 e+06 | AGACA | IPPPPP | −8.66 | −8.54 (0.38) | −0.12 | 11 |
| 5.2 e+07 | AGACA | IPPIPP | −10.41 | −10.09 (0.23) | −0.32 | 5 |
| 9.1 e+07 | AGACA | IPPIPP | −10.74 | −10.09 (0.23) | −0.65 | 5 |
| 8.0 e+06 | ATTCA | IPPIPP | −9.32 | −8.47 (0.42) | −0.85 | 5 |
| 9.2 e+06 | TTACA | IPPIPP | −9.40 | −8.47 (0.42) | −0.93 | 5 |
The predicted free energies and standard errors of the predictions are based on the fit to Model 2. For all of these experiments the temperature is 295 degrees Kelvin. I refers to an imidazole–amide and P refers to a pyrrole–amide in a γ-aminobutyric acid hairpin-linked molecule with a carboxyl-terminal β-alanine–N, N-dimethylaminopropyl-amide tail.