Figure 1.

Saturation digestion of UV-crosslinked RNA–protein complexes with RNase T1. [³²P]-Labeled transcript c–protein complexes were crosslinked with UV irradiation, and the complexes were isolated from a native polyacrylamide gel, subjected to RNase T1 digestion, and resolved in an SDS/15% PAGE. Lane 1, control UV-crosslinked RNA–protein complex without RNase T1 digestion. Lanes 2 and 3, UV–crosslinked complex after digestion with 5 and 50 units of RNase T1, respectively. Arrow points to the fastest migrating RNA–protein complex obtained after digestion with 50 units of RNase T1. Sizes of prestained protein markers are indicated to the right of the gel.