Figure 5.

Recombinant TB-RBP binds to single-stranded DNA oligonucleotides containing specific chromosomal breakpoint sequences. Fifty nanograms of recombinant TB-RBP was incubated with increasing amounts of nonspecific competitor MPR2PRI (5′-CTATAGAATTCTCAAGCTTGC-3′) (lanes 2–4) or specific competitor Bcl-CL1 (lanes 5–7) for 10 min at RT. [³²P]-labeled Bcl-CL1 (0.14 pmol) was then added and incubated for 10 min. The TB-RBP–DNA complex (arrowhead) was detected by a mobility shift assay on a 4% polyacrylamide gel.