Figure 6.

Immunoprecipitation of TB-RBP inhibits the formation of TB-RBP–RNA complexes. (A) Equal amounts of the supernatant from testicular cytoplasmic extracts (20 μg) incubated with TBS (lane 1), pre-immune serum (lane 2), or anti-TB-RBP antibody (lane 3) were incubated with [³²P]-labeled transcript c and the RNA–protein complexes were resolved on 4% native polyacrylamide gels. The TB-RBP–RNA complex is indicated by a solid arrowhead. The p48/52–RNA complex is indicated by an open arrowhead. (B) Western blot assays. Equal volumes (14 μl) of the supernatants used in A were resolved on an SDS/10% polyacrylamide gel, transferred to a nitrocellulose filter, and TB-RBP was detected with antibody against a TB-RBP peptide. TB-RBP is indicated by a solid arrowhead.