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. Author manuscript; available in PMC: 2008 Oct 11.
Published in final edited form as: Cell Host Microbe. 2007 Oct 11;2(4):240–249. doi: 10.1016/j.chom.2007.09.001

Figure 4.

Figure 4

Peptide from the C-terminal portion of PB1-F2 elicits an inflammatory response and primes for secondary bacterial pneumonia. Groups of 5 mice were given peptides or PBS as a control intranasally on day -1 then challenged with 100 CFU of bacteria 24 hours later. (A) Weight loss and (B) mortality are compared. The peptide from the C-terminal region of the PB1-F2 protein caused more weight loss prior to and after bacterial challenge (* p < 0.05 by Student’s t-test) and less survival (* p < 0.01 by log-rank test on the Kaplan-Meier survival data) than did a peptide from the N-terminal region or PBS. Groups of 5 mice were assayed for number of (C) neutrophils and (D) macrophages from broncho-alveolar lavage fluid samples 3 days after exposure to peptide alone. The 25th – 75th percentiles are represented by the shaded box-plots with the horizontal bar indicating the mean value. Error bars indicate the standard deviation of the measurements. An asterisk (*) indicates a significant difference (p < 0.05) by ANOVA compared to both controls at that timepoint.