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. Author manuscript; available in PMC: 2008 Oct 11.
Published in final edited form as: Cell Host Microbe. 2007 Oct 11;2(4):240–249. doi: 10.1016/j.chom.2007.09.001

Figure 7.

Figure 7

1918 PB1-F2 and secondary bacterial pneumonia. Groups of 10 mice were infected with 50 TCID50 of wt PR8 or PR8-PB1-F21918 followed 7 days later by bacterial challenge. (A) Survival following bacterial challenge is plotted, an asterisk (*) indicates a significant difference in survival by the log-rank test on the Kaplan-Meier data. (B) Bacterial lung loads from 5 mice per group and timepoint were determined 24, 48, and 72 hours after bacterial challenge. The 25th – 75th percentiles are represented by the shaded box-plots with the horizontal bar indicating the mean value. Error bars indicate the standard deviation of the measurements. An asterisk (*) indicates a significant difference (p < 0.05) by ANOVA compared to wt PR8 at that timepoint. Lungs were taken from 5 mice per group with evidence of pneumonia by bioluminescence 3 days after infection with either wt PR8 (data not shown) or PB8-PB1-F21918 (C, D). Pictured are representative sections at either 10X (C) or 40X (D) magnification. Massive, lobar, coagulative necrosis with pleuritis and leukocytic infiltration are evident.