Table 1. Plating efficiency of sorted cells.

Two hundred cells were plated at 2 and 4 weeks after sorting and the number of colonies was determined after one week, at 3 and 5 weeks, respectively. The colonies (total of 200) were detected by staining with 0.5% methylene blue in 50% methanol. The difference between the number of colonies in the eGFP-positive and eGFP-negative populations is statistically significant (t-test, p<0.001) at both the three-week and five-week time point.

	eGFP-positive	eGFP-negative
3 weeks	13.5%	43.85%
5 weeks	64.25%	76.65%