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. Author manuscript; available in PMC: 2008 Jul 12.
Published in final edited form as: Cell Host Microbe. 2007 Jul 12;2(1):55–67. doi: 10.1016/j.chom.2007.06.002

Figure 6. CR3 mediates β-1,6-glucan stimulation of neutrophils.

Figure 6

Polybead polystyrene 6 μm microspheres (beads) were coated with the β-1,6-glucan pustulan (pus). Beads were opsonized with pooled human serum and cultured with neutrophils for 15 minutes. Neutrophils were preincubated with CR3 blocking antibodies or IgG isotype control for 30 minutes on ice before culturing with pustulan- coated beads. (A) CR3 blocking antibodies reduce β-1,6-glucan stimulated phagocytosis. Phagocytosis of pustulan- coated beads was assessed by FACS by the change in side scatter for neutrophils preincubated with: (a) isotype control IgG, or (b) anti CR3 blocking antibodies. (B) CR3 blocking antibodies reduce β-1,6-glucan stimulated ROS production. ROS production in response to pustulan- coated beads was assayed by FACS using DHR123. Neutrophils were preincubated with isotype control IgG (green) or anti CR3 blocking Ab (red).