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. Author manuscript; available in PMC: 2008 Jul 12.
Published in final edited form as: Cell Host Microbe. 2007 Jul 12;2(1):55–67. doi: 10.1016/j.chom.2007.06.002

Figure 7. β-1,6-glucan is required for efficient phagocytosis of Candida albicans, production of ROS, and expression of HSPs.

Figure 7

Candida albicans cells were heat killed, digested with an endo-β-1,6-glucanase, and opsonized. (A) β-1,6-glucan is required for efficient phagocytosis. Phagocytosis was assessed by Fluorescence Activated Cell Sorting (FACS) by the change in side scatter. (B) β-1,6-glucan is required for efficient ROS production. ROS production was assayed by FACS using DHR123. (C) β-1,6-glucan is required for induction of HSPs. HSPs induction was determined by quantitative real-time PCR. Results for β-1,6-glucanase digested Candida were normalized to undigested Candida. The data represent the average of two experiments with standard deviation.