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. Author manuscript; available in PMC: 2008 Apr 19.
Published in final edited form as: Cell Host Microbe. 2007 Apr 19;1(2):109–119. doi: 10.1016/j.chom.2007.03.001

Figure 2. The HrtAB system is required for staphylococcal heme adaptation.

Figure 2

A) S. aureus wildtype and ΔhrtA were grown O/N in TSB and then subcultured into TSB supplemented with 0, 5, or 10 μM hemin. Growth rates were determined by measuring the absorbance (O.D. 600) at the indicated time points. B) S. aureus wildtype and ΔhrtA were grown O/N in TSB and then subcultured for 2.5 hrs into TSB supplemented with 0, 5, 10 or 20 μM hemin. The cultures were then serially diluted and plated on TSA plates. C) S. aureus wildtype and ΔhrtA were grown O/N in TSB and TSB supplemented with 1 μM hemin (1→) and then subcultured into TSB supplemented with 0 or 10 μM hemin (→0 or →10). Growth rates were determined by measuring the absorbance (O.D. 600) at the indicated time points (hrs). D) S. aureus wildtype and ΔhrtA transformed with vector alone (WT/pOS and ΔhrtA/pOS) and ΔhrtA transformed with vector containing a wildtype copy of hrtA (ΔhrtA/phrtA) were grown in TSB supplemented with 1 μM hemin and then subcultured into TSB supplemented with +/− 10 μM hemin and grown for 15 hrs. The results represent the mean ± S.D. from triplicate experiments. Asterisks denote statistically significant differences as determined by Student's t test (p≤0.05).