Figure 5.

Effect of VPS41 deletion on Fet3p activity. (a) PPD oxidase activity of wild-type, vps41Δ2, and fet3Δ cells. Five milligrams of a crude membrane fraction obtained from cells grown in low-iron medium was incubated with 0.05% PPD in 100 mM NaOAc (pH 5.7) for 12 hr and quantified by A₅₇₀. (b) Metal-dependent growth phenotypes of vps41Δ1. The effect of added iron or copper on growth on LIM medium was determined for the indicated yeast strains. A, 5 μM Fe, 0.125 μM Cu; B, 5 μM Fe, 500 μM Cu; C, 50 μM Fe, 500 μM Cu.