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. Author manuscript; available in PMC: 2008 Oct 12.
Published in final edited form as: Mol Cell. 2007 Oct 12;28(1):68–78. doi: 10.1016/j.molcel.2007.07.027

Figure 3. CUGBP1 is hyper-phosphorylated in heart tissue from an inducible DM1 mouse model.

Figure 3

(A) Heart tissue from bitransgenic animals in which DMPK-CUG960 mRNA expression was induced (EpA960/MCM +Tamoxifen) or not induced (EpA960/MCM + mock) were analyzed by 2D/western blotting. MCM transgenic animals injected with tamoxifen (MCM +Tamoxifen) do not exhibit CUGBP1 hyper-phosphorylation. The results were reproduced in at least three different bitransgenic and transgenic animals. (B) Timing of CUGBP1 hyper-phosphorylation directly correlates with elevated CUGBP1 protein levels. A longer exposure was used for the 6h sample to better detect acidic shifts of CUGBP1.