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. Author manuscript; available in PMC: 2008 Oct 12.
Published in final edited form as: Mol Cell. 2007 Oct 12;28(1):68–78. doi: 10.1016/j.molcel.2007.07.027

Figure 7. CUGBP1 is hyper-phosphorylated in newborn and embryonic heart and skeletal muscle tissues.

Figure 7

CUGBP1 phosphorylation was analyzed on 2D gels (A) during heart development (e17, newborn and 6 months old adult mice), and (B) during skeletal muscle development (newborn and 6 months old adult mice) by western blot using HRP conjugated CUGBP1 monoclonal antibody. Seventy-five micrograms of total protein from e17 and newborn tissues and one hundred fifty micrograms of adult heart tissues were analyzed. Representative results from at least three independent experiments are shown. Note that e17 heart samples were a pool of eight mice hearts. (C) CUGBP1 is hyper-phosphorylated in newborn heart. Untreated, mock treated or CIAP-treated newborn heart tissues were analyzed by 2D/western blotting.