Table 1.
Chromatographic results obtained on the Chirabiotic T column for the enantioselective separation of (R,S)-IF, (R,S)-2-DCl-IF and (R,S)-3-DCl-IF; where: k2 = the retention of the second eluting enantiomer, α = the enantioselectivity, Rs = enantiomeric resolution, MeOH = methanol, IPA = 2-propanol, EtOH = ethanol, ACN = acetonitrile. See text for chromatographic conditions.
| Mobile Phase | Ifosfamide | 2-DCl-IF | 3-DCl-IF | ||||||
|---|---|---|---|---|---|---|---|---|---|
| k2 | α | Rs | k2 | α | Rs | k2 | α | Rs | |
| MeOH | 2.2 | 1.3 | 1.5 | 4.0 | 1.3 | 2.2 | 5.2 | 1.2 | 1.8 |
| IPA | 10.3 | 1.9 | 1.9 | 26 | 1.6 | 4.4 | 40 | 1.4 | 2.4 |
| ACN | 4.1 | 1.0 | - | 1.5 | 1.0 | - | 1.5 | 1.0 | - |
| ACN:IPA (30:70) |
1.7 | 1.1 | 0.73 | 2.8 | 1.0 | 0.70 | 3.5 | 1.1 | 0.90 |
| MeOH:IPA (35:65) |
2.3 | 1.2 | 1.6 | 3.7 | 1.2 | 2.6 | 5.1 | 1.2 | 1.9 |
| MeOH:IPA (40:60) |
2.0 | 1.2 | 1.8 | 3.6 | 1.2 | 2.6 | 4.6 | 1.2 | 1.9 |
| EtOH:IPA (50:50) |
3.9 | 1.5 | 1.9 | 8.0 | 1.4 | 1.8 | 11.1 | 1.3 | 1.7 |
| EtOH:IPA (40:60) |
4.2 | 1.5 | 2.2 | 6.1 | 1.4 | 1.8 | 12.9 | 1.3 | 2.0 |
| EtOH:IPA (70:30) |
2.9 | 1.3 | 1.9 | 5.7 | 1.3 | 1.9 | 7.7 | 1.2 | 1.8 |