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. Author manuscript; available in PMC: 2008 Oct 19.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Aug 13;362(2):288–294. doi: 10.1016/j.bbrc.2007.07.186

Figure 2. Transcriptional regulation of fibronectin by TGFβ in mesangial cells.

Figure 2

A) Mesangial cells stably transfected with a fibronectin-luciferase (fibro-luc) reporter construct were treated with increasing amounts of TGFβ. Fibronectin promoter activity was determined by luciferase measurement and normalized by protein concentration. Data shown are the mean +/− SEM of triplicate samples. * p<0.05 ** p<0.01 B) Fibro-luc mesangial cells were treated with increasing amounts of CsA prior to addition of TGFβ (2ng/ml). Fibronectin promoter activity was determined by measurement of luciferase normalized by protein concentration. Data shown are the mean +/− SEM of triplicate samples. *p<0.05 **p<0.01 C) Mesangial cells were transiently transfected with increasing amounts of constitutively active calcineurin for 72 hours. Cells were lysed, total protein collected and separated by SDS-PAGE. Fibronectin was detected using a primary antibody specific for fibronectin. Tubulin expression was also determined as an internal control. Results of a typical experiment were semi-quantitated and graphed. Data shown are representative of 3 independent experiments. D) Fibro-luc mesangial cells were transiently transfected with increasing amounts of constitutively active calcineurin. Fibronectin promoter activity was determined by measurement of luciferase normalized by protein concentration. Data shown are the mean +/− SEM of triplicate samples. *p<0.05, ** p<0.01, #