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. Author manuscript; available in PMC: 2008 May 21.
Published in final edited form as: Sens Actuators B Chem. 2007 May 21;123(2):784–792. doi: 10.1016/j.snb.2006.10.019

Fig. 2.

Fig. 2

A representative SPR sensorgram corresponding to the incorporation of Cd2+ (0.13 μM) by immobilized apo-MT (curve a). Prior to the incorporation of Cd2+, the pristine metals in the immobilized MT molecules were first released by injecting a glycine–HCl buffer solution (sensorgram shown in the inset). Curve b was acquired in a similar fashion as curve a except that an Ala5-modified Au sensor chip was used. Degassed, boiled water was used as the carrier solution. A flow rate of 1 mL/h and a 50-μL sample loop were used. The arrow indicates the time when the injection was made.