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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Cell Metab. 2007 Aug;6(2):105–114. doi: 10.1016/j.cmet.2007.07.003

Figure 2.

Figure 2

Sirt2 knockdown promotes 3T3L1 adipocyte differentiation. Stable shRNA transfected 3T3-L1 preadipocytes were subjected to differentiation using the standard protocol. Oil Red O staining of shGFP and shSirt2 cells on day 4 of differentiation indicated that shSirt2 had accelerated differentiation with enhanced lipid staining. During 3T3-L1 adipocyte differentiation, shSirt2 cells (empty circles) had consistently lower endogenous Sirt2 mRNA expression compared with shGFP cells (solid circles). The mRNA expression for various differentiation markers was also determined by realtime PCR. The protein expression of different adipocyte differentiation markers was determined by Western blotting.