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. Author manuscript; available in PMC: 2008 Sep 7.
Published in final edited form as: Mol Cell. 2007 Sep 7;27(5):731–744. doi: 10.1016/j.molcel.2007.06.033

Table 1.

Mutagenesis to remove PAN’s C–terminal arginine destroys its ability to stimulate gate opening, but its replacement by certain other residues allows wild type activity

PAN Sequence Peptide Hydrolysis (% WT)
AHLDVLYR430 (WT) 100
AHLDVLY- 4
AHLDVLYD 5
AHLDVLYA 100
AHLDVLYW 106
AHLDVLYL 13
AHLDVLYG 77
AHLDVLYRA 2
AAAAAHLDVLYR 74
No PAN 5

PANs C-terminal residue was mutated and the indicated PAN mutant (1ug) was incubated with 20S (0.25ug) in a 100ml reaction buffer and the rate of LFP hydrolysis was monitored in the presence or absence of ATPgS (20mM). Shown is the mean values of 3 or more experiments were the +/−SD was less than 5%.