Table 1.
PAN Sequence | Peptide Hydrolysis (% WT) |
---|---|
AHLDVLYR430 (WT) | 100 |
AHLDVLY- | 4 |
AHLDVLYD | 5 |
AHLDVLYA | 100 |
AHLDVLYW | 106 |
AHLDVLYL | 13 |
AHLDVLYG | 77 |
AHLDVLYRA | 2 |
AAAAAHLDVLYR | 74 |
No PAN | 5 |
PANs C-terminal residue was mutated and the indicated PAN mutant (1ug) was incubated with 20S (0.25ug) in a 100ml reaction buffer and the rate of LFP hydrolysis was monitored in the presence or absence of ATPgS (20mM). Shown is the mean values of 3 or more experiments were the +/−SD was less than 5%.