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Competition of GST-Tn.2345–2541 for talin F-actin binding activity. Rabbit actin (10 μM) and talin (1 μM) were mixed in the presence of increasing amounts of GST-Tn (0–20 μM), and actin polymerization, centrifugation, SDS/PAGE, and densitometry were performed as previously described (Fig. 2). Values reported are averages of three separate experiments. GST (0–20 μM) had no effect on talin binding to F-actin (not shown).
