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Nucleation of F-actin polymerization. Actin (6 μM, 54% pyrene-labeled) was polymerized alone (○) or in the presence of talin (2 μM, ▿), GST-Tn, GST-Sla2, or GST (2 μM each (•), or gelsolin (2 nM, ▾) and polymerization was monitored by the increase in pyrene fluorescence.
