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Schematic illustration of sensitized emission resonance energy transfer between antibody-labeled dystrophin and tetramethylrhodamine phalloidin-labeled actin. The steps in the protocol (Top) and the two possible results of the experiment and their interpretations (Middle and Bottom) are shown. Sensitized emission of the tetramethylrhodamine phalloidin-labeled actin occurs only if the labeled dystrophin is within about 10 nm from the labeled actin. Protein sizes are not drawn to scale.
