Table 1.

SEIRET on paraformaldehyde-fixed and unfixed tissue sections

Muscle section	τD547, ms	τDA547, ms	τDA568, ms	E	EavgDQ	EavgL	FDQ	FL
Fixed	2.0  ±  0.3	1.2  ±  0.1	0.7  ±  0.1	0.65	0.4	0.4	0.6	0.6
Unfixed	1.8  ±  0.1	0.8  ±  0.1	0.59  ±  0.04	0.67	0.6	0.56	0.9	0.83

τ_D547, donor only lifetime at 547 mm; τ_DA547, donor plus acceptor lifetime at 547 nm; τ_DA568, donor plus acceptor lifetime at 568 nm; E, efficiency of energy transfer; E_avgDQ, average energy transfer by donor quenching; E_avgL, average energy transfer by lifetime method; F_DQ, fraction of coupled donors by donor quenching; F_L, fraction of coupled donors by lifetime method.