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. Author manuscript; available in PMC: 2007 Nov 21.
Published in final edited form as: Free Radic Biol Med. 2007 Jul 3;43(9):1313–1321. doi: 10.1016/j.freeradbiomed.2007.06.015

Figure 3. Doxorubicin-induced inhibition of protein synthesis occurs at the elongation step of translation.

Figure 3

(A) Polysome analysis of untreated cells and cells treated with 250 ng/ml doxorubicin for 20 hours. (B) Western blot analysis of untreated cells and cells treated with 250 ng/ml doxorubicin using antibodies against phosphorylated EF-2 (P-EF-2), total EF-2 (EF-2) and EF-2 kinase (EF-2K). Note: The EF-2-P membrane was stripped and reprobed for EF-2K. The upper band on the EF-2K membrane is residual EF-2-P signal. Actin serves as a loading control.