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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Immunity. 2007 Aug;27(2):253–267. doi: 10.1016/j.immuni.2007.07.012

Figure 3. Altered antibody responses in B-TRAF3-/- mice.

Figure 3

(A) Basal serum levels of Ig isotypes. Sera from naïve LMC and B-TRAF3-/- mice (n=10 for each group) were tested for IgM, IgG1, IgG2a, IgG2b, IgG3, and IgE by ELISA. Mice analyzed were 10 to 12 weeks old. (B) T-I antibody responses. Mice (8-10 weeks old, n=10 for each group) were immunized with the T-I Ag TNP-Ficoll/Alum, and sera were collected on day 10 after immunization. Serum levels of anti-TNP IgM, IgG1, IgG2a, IgG2b and IgG3 were analyzed by ELISA. (C) T-D antibody responses. Mice (8-10 weeks old, n=11 for each group) were immunized with the T-D Ag TNP-KLH/Alum, and boosted on day 21 after the first immunization. Sera were collected on day 7, 14 and 28 after the first immunization. Serum levels of anti-TNP IgM and IgG1 were measured by ELISA. Multiple serial dilutions of each serum sample were tested to ensure the readout is within the linear range of the assay. Relative titer = A405 value × 10 × Dilution factor. Anti-TNP specific Ig levels in pre-immune sera (with 1:100 dilution) of both littermate control and B-TRAF3-/- mice were below the detection limit of ELISA. *, significantly different from LMC (t-test, P<0.05); **, very significantly different from LMC (t-test, P<0.01).