Table 8. Coverage of genes associated with metabolic pathways in rice FL-cDNAs libraries.
Pathway id (a) | Pathway name (a) | All-CDS | CDS-AE | CDS-ANE | CDS-AE proportion (c) | Significance (d) |
PWY-2881,2901, 2902 (b) | cytokinins -glucoside biosynthesis | 235 | 144 | 91 | 61.3 | Low |
LIPAS-PWY | triacylglycerol degradation | 177 | 127 | 50 | 71.8 | Low |
PWY-1081 | homogalacturonan degradation | 134 | 95 | 39 | 70.9 | Low |
DETOX1-PWY | removal of superoxide radicals | 79 | 13 | 66 | 16.5 | Low |
GLUCONEO-PWY | gluconeogenesis | 67 | 61 | 6 | 91.0 | High |
PWY-3781 | aerobic respiration | 66 | 39 | 27 | 59.1 | Low |
TRNA-CHARGING-PWY | tRNA charging pathway | 64 | 61 | 3 | 95.3 | High |
P61-PWY | UDP-glucose conversion | 63 | 60 | 3 | 95.2 | High |
GALACTMETAB-PWY | galactose degradation I | 54 | 53 | 1 | 98.1 | High |
COLANSYN-PWY | colanic acid building blocks biosynthesis | 51 | 50 | 1 | 98.0 | High |
PWY-1121 | suberin biosynthesis | 51 | 34 | 17 | 66.7 | Low |
PWY-3821 | galactose degradation III | 51 | 51 | 0 | 100.0 | High |
PWY1F-FLAVSYN | flavonoid biosynthesis | 50 | 24 | 26 | 48.0 | Low |
CHLOROPHYLL-SYN | chlorophyll biosynthesis | 48 | 46 | 2 | 95.8 | High |
P1-PWY | salvage pathways of purine and pyrimidine nucleotides | 44 | 42 | 2 | 95.5 | High |
PWY-1861 | formaldehyde assimilation II (RuMP Cycle) | 42 | 40 | 2 | 95.2 | High |
PWY-2181 | phenylpropanoid biosynthesis | 40 | 23 | 17 | 57.5 | Low |
Metabolic pathway-related CDS | 2750 | 2125 | 625 | 77.3 |
: The classification was based on the RiceCyc database at the GRAMENE Web site (http://www.gramene.org/pathway/).
: PWY-2881, PWY-2901, and PWY-2902 share the same genes in the respective pathways.
: The ratio was calculated as the number of CDS-AE loci/total number of loci.
: Significances of difference were examined between the CDS-AE proportion in genes in the respective pathway and that of all genes associated with metabolic pathways by the chi-squared test. High (Low) represents that the cloning efficiency of genes for a metabolic pathway was higher (lower) than the collection efficiency for all genes associated with metabolic pathways.