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Essential role of Trp-572 side chain in the metal ion protective effect. Protection of pigeon c-NADP-ME (A and B, WT; C and D, W572A; E and F, W572F; G and H, W572H; I and J, W572I; and K and L, W572S) by Mn2+ against urea-induced aggregation was monitored by average emission wavelength (panels A, C, E, G, I, and K) or CD (B, D, F, H, J, and L). (Open circles) in the absence of Mn2+; (solid circles) in the presence of 4 mM Mn2+.
