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ANS binding of pigeon c-NADP-ME during urea denaturation. The exposure of the hydrophobic regions of the enzyme in urea was examined using the integral fluorescence area in the presence of ANS; this was done either in the absence of Mn2+ (open circles, WT; open triangles, W572F) or in the presence of 4 mM Mn2+ (solid circles, WT; solid triangles, W572F).
