FIGURE 1.

(A) Sketch of the experimental setup for monitoring in vitro mobility. Confluent IEC-6 cells were plated on glass coverslips, scraped with a cell scraper, and then mounted on the stage of an Olympus 1X71 (Tokyo, Japan) inverted microscope warmed to 37°C. Fresh medium was continuously perfused across the cells. Differential interference contrast images were obtained every 5 min. (B) Schematic representation of the cell layer as one-dimensional continuum (only one side of the wound is shown): i), initial state; ii), hypothetical state at time t accounting for growth but not deformation; iii), true configuration of the layer at time t.