TABLE 5.
Parameters of the best-fit models for wild-type and mutant human IgG3s and IgG4s (39)
| Model | θ (degree) | ϕ (degree) | Projection on Z axis (Å) | Hinge distance between Fab and Fc (Å) | Angle between Fabs | Dmax (Å) |
|---|---|---|---|---|---|---|
| (a) Wild-type IgG3 | ||||||
| Fc arm | 90.0 | 0 | −88.2 | — | 80.2 | 196 |
| Fab1 arm | 111.9 | 243.6 | −8.7 | 79.5 | ||
| Fab2 arm | 99.4 | 326.8 | −3.8 | 84.4 | ||
| (b) IgG3m15 | ||||||
| Fc arm | 90.0 | 0 | −19.8 | — | 108.0 | 163 |
| Fab1 arm | 64.0 | 17.9 | 3.9 | 23.7 | ||
| Fab2 arm | 69.0 | 254.2 | 3.2 | 23.0 | ||
| (c) IgG3HM5 | ||||||
| Fc arm | 90.0 | 0 | −1.8 | — | 108.0 | 164 |
| Fab1 arm | 64.0 | 17.9 | 7.1 | 8.9 | ||
| Fab2 arm | 69.0 | 254.2 | 5.8 | 7.6 | ||
| (d) Wild-type IgG4 model A with 2-2-2 hinge | ||||||
| Fc arm | 90.0 | 0 | −9.0 | — | 122.4 | 162 |
| Fab1 arm | 65.6 | 176.3 | 3.72 | 12.72 | ||
| Fab2 arm | 57.4 | 4.6 | 4.85 | 13.85 | ||
| (e) Wild-type IgG4 model B with 2-2-3 hinge | ||||||
| Fc arm | 90.0 | 0 | −12.6 | — | 122.4 | 162 |
| Fab1 arm | 65.6 | 176.3 | 3.72 | 16.32 | ||
| Fab2 arm | 57.4 | 4.6 | 4.85 | 17.45 | ||
| (f) IgG4S331P mutant model A | ||||||
| Fc arm | 90.0 | 0 | −12.6 | — | 108.0 | 160 |
| Fab1 arm | 64.0 | 17.9 | 5.52 | 18.12 | ||
| Fab2 arm | 69.0 | 254.2 | 4.52 | 17.12 | ||
| (g) IgG4S331P mutant model B | ||||||
| Fc arm | 90.0 | 0 | −12.5 | — | 108.0 | 160 |
| Fab1 arm | 64.0 | 17.9 | 5.17 | 17.67 | ||
| Fab2 arm | 69.0 | 254.2 | 4.23 | 16.73 | ||