Fig. 3.

Effect of chemical and biochemical pretreatments on binding of P2-GFP to maxillary stylets. Stylets were pretreated with the compounds indicated before P2-GFP incubation and observation by epifluorescence microscopy. (A and B) Extraction of polar lipids (A) and nonpolar lipids (B) with n-hexane and chloroform:methanol, respectively. (C) Sodium periodate oxidization of carbohydrate compounds: all treatments described in Materials and Methods gave identical images. (D–F) Digestion with various proteinases as indicated. (G–J) Effect of pretreatment with chitinase with or without subtilisin. (G) Long chitinase digestion alone. (H) Short chitinase digestion alone. (I) Subtilisin digestion alone (control). (J) Short chitinase digestion followed by subtilisin digestion. (Scale bars: 5 μm.)