Single garlic-derived organic polysulfide-induced (nonenzymatic) H2S production by GSH. (A) Representative polarographic traces of H2S production in anoxic 10 mM PBS with 50 μM DTPA, with 100 μM each DATS (red line), DADS (green line), DAS (brown line), AMS (yellow line), and DPDS (blue line), by addition of 2 mM GSH at arrow. (B) Total H2S yield from each compound under the same conditions as in A; each bar represents the mean ± SD of three to six experiments. *, Yields are statistically different compared with yield from DPDS by Student's t test (P ≤ 0.005).